An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in Staphylococcus aureus.
Identifieur interne : 000B37 ( Main/Exploration ); précédent : 000B36; suivant : 000B38An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in Staphylococcus aureus.
Auteurs : Raid Al Akeel [Arabie saoudite] ; Ayesha Mateen [Arabie saoudite] ; Rabbani Syed [Arabie saoudite]Source :
- Journal of AOAC International [ 1944-7922 ] ; 2019.
Descripteurs français
- KwdFr :
- Biofilms (effets des médicaments et des substances chimiques), Détection du quorum (effets des médicaments et des substances chimiques), Facteurs de virulence (métabolisme), Hémolysines (métabolisme), Polyosides bactériens (métabolisme), Populus (composition chimique), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines végétales (pharmacologie), Régulation négative (MeSH), Staphylococcus aureus (effets des médicaments et des substances chimiques), Staphylococcus aureus (pathogénicité), Staphylococcus aureus (physiologie), Virulence (effets des médicaments et des substances chimiques).
- MESH :
- composition chimique : Populus.
- effets des médicaments et des substances chimiques : Biofilms, Détection du quorum, Staphylococcus aureus, Virulence.
- génétique : Protéines bactériennes.
- métabolisme : Facteurs de virulence, Hémolysines, Polyosides bactériens, Protéines bactériennes.
- pathogénicité : Staphylococcus aureus.
- pharmacologie : Protéines végétales.
- physiologie : Staphylococcus aureus.
- Régulation négative.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Biofilms (drug effects), Down-Regulation (MeSH), Hemolysin Proteins (metabolism), Plant Proteins (pharmacology), Polysaccharides, Bacterial (metabolism), Populus (chemistry), Quorum Sensing (drug effects), Staphylococcus aureus (drug effects), Staphylococcus aureus (pathogenicity), Staphylococcus aureus (physiology), Virulence (drug effects), Virulence Factors (metabolism).
- MESH :
- chemical , genetics : Bacterial Proteins.
- chemical , metabolism : Bacterial Proteins, Hemolysin Proteins, Polysaccharides, Bacterial, Virulence Factors.
- chemistry : Populus.
- drug effects : Biofilms, Quorum Sensing, Staphylococcus aureus, Virulence.
- pathogenicity : Staphylococcus aureus.
- chemical , pharmacology : Plant Proteins.
- physiology : Staphylococcus aureus.
- Down-Regulation.
Abstract
Background: Alanine-rich proteins/peptides (ARP), with bioactivity of up to 20 amino acid residues, can be observed by the body easily during gastrointestinal digestion. Objective: Populus trichocarpa extract's capability to attenuate quorum sensing-regulated virulence and biofilm formation in Staphylococcus aureus is described. Methods: PT13, an ARP obtained from P. trichocarpa, was tested for its activity against S. aureus using the broth microdilution test; a crystal-violet biofilm assay was performed under a scanning electron microscope. The production of various virulence factors was estimated with PT13 treatment. Microarray gene expression profiling of PT13-treated S. aureus was conducted and compared with an untreated control. Exopolysaccharides (EPS) was estimated to observe the PT13 inhibition activity. Results: PT13 was antimicrobial toward S. aureus at different concentrations and showed a similar growth rate in the presence and absence of PT13 at concentrations ≤8 μg/mL. Biofilm production was interrupted even at low concentrations, and biofilm-related genes were down-regulated when exposed to PT13. The genes encoding cell adhesion and bacterial attachment protein were the major genes suppressed by PT13. In addition, hemolysins, clumping activity, and EPS production of S. aureus decreased after treatment in a concentration-dependent manner. Conclusions: A long-chain PT13 with effective actions that, even at low concentration levels, not only regulated the gene expression in the producer organism but also blocked the virulence gene expression in this Gram-positive human pathogen is described. Highlights: We identified a PT13 as a potential antivirulence agent that regulated production of bacterial virulence determinants (e.g., toxins, enzymes and biofilm), downwards and it may be a promising anti-virulence agent to be further developed as an anti-infective agent.
DOI: 10.5740/jaoacint.18-0251
PubMed: 30446019
Affiliations:
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University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Saud University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Mateen, Ayesha" sort="Mateen, Ayesha" uniqKey="Mateen A" first="Ayesha" last="Mateen">Ayesha Mateen</name><affiliation wicri:level="1"><nlm:affiliation>King Saud University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Saud University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author><author><name sortKey="Syed, Rabbani" sort="Syed, Rabbani" uniqKey="Syed R" first="Rabbani" last="Syed">Rabbani Syed</name><affiliation wicri:level="1"><nlm:affiliation>King Saud University, College of Pharmacy, Department of Pharmaceutics, PO Box 2457, Riyadh 11433, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Saud University, College of Pharmacy, Department of Pharmaceutics, PO Box 2457, Riyadh 11433</wicri:regionArea><wicri:noRegion>Riyadh 11433</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of AOAC International</title><idno type="eISSN">1944-7922</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Biofilms (drug effects)</term><term>Down-Regulation (MeSH)</term><term>Hemolysin Proteins (metabolism)</term><term>Plant Proteins (pharmacology)</term><term>Polysaccharides, Bacterial (metabolism)</term><term>Populus (chemistry)</term><term>Quorum Sensing (drug effects)</term><term>Staphylococcus aureus (drug effects)</term><term>Staphylococcus aureus (pathogenicity)</term><term>Staphylococcus aureus (physiology)</term><term>Virulence (drug effects)</term><term>Virulence Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biofilms (effets des médicaments et des substances chimiques)</term><term>Détection du quorum (effets des médicaments et des substances chimiques)</term><term>Facteurs de virulence (métabolisme)</term><term>Hémolysines (métabolisme)</term><term>Polyosides bactériens (métabolisme)</term><term>Populus (composition chimique)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines végétales (pharmacologie)</term><term>Régulation négative (MeSH)</term><term>Staphylococcus aureus (effets des médicaments et des substances chimiques)</term><term>Staphylococcus aureus (pathogénicité)</term><term>Staphylococcus aureus (physiologie)</term><term>Virulence (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Hemolysin Proteins</term><term>Polysaccharides, Bacterial</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biofilms</term><term>Quorum Sensing</term><term>Staphylococcus aureus</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Biofilms</term><term>Détection du quorum</term><term>Staphylococcus aureus</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de virulence</term><term>Hémolysines</term><term>Polyosides bactériens</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation négative</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Background:</i> Alanine-rich proteins/peptides (ARP), with bioactivity of up to 20 amino acid residues, can be observed by the body easily during gastrointestinal digestion. <i>Objective: Populus trichocarpa</i> extract's capability to attenuate quorum sensing-regulated virulence and biofilm formation in <i>Staphylococcus aureus</i> is described. <i>Methods:</i> PT13, an ARP obtained from <i>P. trichocarpa</i>, was tested for its activity against <i>S. aureus</i> using the broth microdilution test; a crystal-violet biofilm assay was performed under a scanning electron microscope. The production of various virulence factors was estimated with PT13 treatment. Microarray gene expression profiling of PT13-treated <i>S. aureus</i> was conducted and compared with an untreated control. Exopolysaccharides (EPS) was estimated to observe the PT13 inhibition activity. <i>Results:</i> PT13 was antimicrobial toward <i>S. aureus</i> at different concentrations and showed a similar growth rate in the presence and absence of PT13 at concentrations ≤8 μg/mL. Biofilm production was interrupted even at low concentrations, and biofilm-related genes were down-regulated when exposed to PT13. The genes encoding cell adhesion and bacterial attachment protein were the major genes suppressed by PT13. In addition, hemolysins, clumping activity, and EPS production of <i>S. aureus</i> decreased after treatment in a concentration-dependent manner. <i>Conclusions:</i> A long-chain PT13 with effective actions that, even at low concentration levels, not only regulated the gene expression in the producer organism but also blocked the virulence gene expression in this Gram-positive human pathogen is described. <i>Highlights:</i> We identified a PT13 as a potential antivirulence agent that regulated production of bacterial virulence determinants (e.g., toxins, enzymes and biofilm), downwards and it may be a promising anti-virulence agent to be further developed as an anti-infective agent.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30446019</PMID><DateCompleted><Year>2020</Year><Month>01</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>01</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1944-7922</ISSN><JournalIssue CitedMedium="Internet"><Volume>102</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Journal of AOAC International</Title><ISOAbbreviation>J AOAC Int</ISOAbbreviation></Journal><ArticleTitle>An Alanine-Rich Peptide Attenuates Quorum Sensing-Regulated Virulence and Biofilm Formation in <i>Staphylococcus aureus</i>.</ArticleTitle><Pagination><MedlinePgn>1228-1234</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5740/jaoacint.18-0251</ELocationID><Abstract><AbstractText><i>Background:</i> Alanine-rich proteins/peptides (ARP), with bioactivity of up to 20 amino acid residues, can be observed by the body easily during gastrointestinal digestion. <i>Objective: Populus trichocarpa</i> extract's capability to attenuate quorum sensing-regulated virulence and biofilm formation in <i>Staphylococcus aureus</i> is described. <i>Methods:</i> PT13, an ARP obtained from <i>P. trichocarpa</i>, was tested for its activity against <i>S. aureus</i> using the broth microdilution test; a crystal-violet biofilm assay was performed under a scanning electron microscope. The production of various virulence factors was estimated with PT13 treatment. Microarray gene expression profiling of PT13-treated <i>S. aureus</i> was conducted and compared with an untreated control. Exopolysaccharides (EPS) was estimated to observe the PT13 inhibition activity. <i>Results:</i> PT13 was antimicrobial toward <i>S. aureus</i> at different concentrations and showed a similar growth rate in the presence and absence of PT13 at concentrations ≤8 μg/mL. Biofilm production was interrupted even at low concentrations, and biofilm-related genes were down-regulated when exposed to PT13. The genes encoding cell adhesion and bacterial attachment protein were the major genes suppressed by PT13. In addition, hemolysins, clumping activity, and EPS production of <i>S. aureus</i> decreased after treatment in a concentration-dependent manner. <i>Conclusions:</i> A long-chain PT13 with effective actions that, even at low concentration levels, not only regulated the gene expression in the producer organism but also blocked the virulence gene expression in this Gram-positive human pathogen is described. <i>Highlights:</i> We identified a PT13 as a potential antivirulence agent that regulated production of bacterial virulence determinants (e.g., toxins, enzymes and biofilm), downwards and it may be a promising anti-virulence agent to be further developed as an anti-infective agent.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Al Akeel</LastName><ForeName>Raid</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>King Saud University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mateen</LastName><ForeName>Ayesha</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>King Saud University, College of Applied Medical Sciences, Department of Clinical Laboratory Sciences, PO Box 10219, Riyadh 11433, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Syed</LastName><ForeName>Rabbani</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>King Saud University, College of Pharmacy, Department of Pharmaceutics, PO Box 2457, Riyadh 11433, Saudi Arabia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J AOAC Int</MedlineTA><NlmUniqueID>9215446</NlmUniqueID><ISSNLinking>1060-3271</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011135">Polysaccharides, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018441" MajorTopicYN="N">Biofilms</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011135" MajorTopicYN="N">Polysaccharides, Bacterial</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053038" MajorTopicYN="N">Quorum Sensing</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30446019</ArticleId><ArticleId IdType="doi">10.5740/jaoacint.18-0251</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Al Akeel, Raid" sort="Al Akeel, Raid" uniqKey="Al Akeel R" first="Raid" last="Al Akeel">Raid Al Akeel</name></noRegion><name sortKey="Mateen, Ayesha" sort="Mateen, Ayesha" uniqKey="Mateen A" first="Ayesha" last="Mateen">Ayesha Mateen</name><name sortKey="Syed, Rabbani" sort="Syed, Rabbani" uniqKey="Syed R" first="Rabbani" last="Syed">Rabbani Syed</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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